The identification of high-performing antibodies for Secreted frizzled-related protein 1 (sFRP-1) for use in Western Blot and immunoprecipitation

Secreted frizzled-related protein 1 (sFRP-1) is a secreted protein, belonging to the glycoprotein SFRP family. As modulator of Wnt/β-catenin signalling pathway, sFRP-1 has implications in human cancers and neurologic diseases. If community had access well-characterized anti-sFRP-1 antibodies, reproducibility research would be enhanced. In this study, we characterized 11 commercial antibodies for Western Blot immunoprecipitation, using standardized experimental protocol based on comparing read-outs knockout cell lines isogenic parental controls. We identified many well-performing encourage readers use report as guide select most appropriate antibody their specific needs.